Development of Transgenic Glyphosate-Resistant Rice with G6 Gene Encoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glypbosateresistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from Pseudomonas putida that encoded 5-enolpy...

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Bibliographic Details
Published in:Agricultural sciences in China 2011-09, Vol.10 (9), p.1307-1312
Main Authors: ZHAO, Te, LIN, Chao-yang, SHEN, Zhi-cheng
Format: Article
Language:English
Subjects:
crops
EPSPS
field experimentation
genes
genetic engineering
glyphosate
glyphosate-resistance
nitric oxide synthase
polymerase chain reaction
Pseudomonas putida
rice
seeds
spraying
transgenic plants
transgenic rice
weed control
Western印迹
农杆菌介导转化
合成酶
基因编码
恶臭假单胞菌
抗草甘膦作物
磷酸
转基因水稻
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Summary:Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glypbosateresistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from Pseudomonas putida that encoded 5-enolpyruvylshikimate-3-phosphate synthase （EPSPS） was previously isolated. The G6 gene was transfected into rice via Agrobacterium-mediated transformation. The transgenic rice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmed that the transgenic rice can survive glyphosate spraying at a dose of 8 g L^-1. In contrast, conventional rice was killed at a weed control glyphosate spray dose of 1 g L^-1. Altogether, the present study showed that the G6 gene works well in rice in vivo for glyphosate-resistance.
ISSN:1671-2927
2095-3119
DOI:10.1016/S1671-2927(11)60123-5