Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum

• Published in: Agricultural sciences in China 2011-12, Vol.10 (12), p.1851-1860
• Main Authors: CHEN, Shuai, LIU, Guan-shan, WANG, Yuan-ying, SUN, Yu-he, CHEN, Jia
• Format: Article
• Language: English
• Subjects: abiotic stress; abiotic stress CDPK Nicotiana tabacum RACE real-time qRT-PCR; CDPK; CDPKs; Nicotiana tabacum; RACE; real-time qRT-PCR; 丝氨酸/苏氨酸激酶; 干旱胁迫; 普通烟草; 蛋白激酶基因; 诱导表达; 钙依赖型蛋白激酶; 高盐
• ISSN: 1671-2927; 2095-3119; 2352-3425
• DOI: 10.1016/S1671-2927(11)60185-5

Calcium-dependent protein kinases （CDPKs, EC 2.7.1.37） comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca^2＋ signal transduction. A full-length CDPK gene, NtCDPK12 （GenBank accession number GQ337420）, was isolated from common tobacco （Nicotiana tabacum） leaves by rapid amplification of eDNA ends （RACE）. The NtCDPK12 eDNA is 1 816 bp length and contains an open reading frame （ORF） of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR （qRT- PCR） showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.