Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds

It is important to determine the isoflavone components by high-performance liquid chromatography (HPLC) for the molecular assistant selection of isoflavone in soybean. Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorb...

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Bibliographic Details
Published in:Agricultural sciences in China 2011-01, Vol.10 (1), p.70-77
Main Authors: SUN, Jun-ming, SUN, Bao-li, HAN, Fen-xia, YAN, Shu-rong, YANG, Hua, Akio, Kikuchi
Format: Article
Language:English
Subjects:
absorbance
acetic acid
acetonitrile
daidzein
genistein
Glycine max
glycitein
high performance liquid chromatography
HPLC
isoflavone
seeds
soybean ( Glycine max L. Merri)
soybeans
ultraviolet radiation
光度法测定
分子辅助选择
大豆异黄酮
成分分析
标准样本
线性梯度洗脱
高效液相色谱法
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Summary:It is important to determine the isoflavone components by high-performance liquid chromatography (HPLC) for the molecular assistant selection of isoflavone in soybean. Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. The results showed that there were different maximum ultraviolet (UV) absorbance for the aglycones of daidzein, glycitein, and genistein, which were at 250, 257, and 260 nm, respectively. A linear gradient elution of acetonitrile (13–30%) containing 0. 1% acetic acid as a mobile phase was applied on a YMC-C18 column at 35°C. The 12 isoflavone components were determined using the UV detector by HPLC. We concluded that this is a rapid and precise method which adapted to determine the large numbers of samples with microanalysis.
ISSN:1671-2927
DOI:10.1016/S1671-2927(11)60308-8