Immunological Activities of Components from Leaves of Liriodendron chinensis

• Published in: Chinese herbal medicines 2015-08, Vol.7 (3), p.279-282
• Main Authors: Yang, Nian-yun, Wang, Liu-ying, Zhang, Yi-wen
• Format: Article
• Language: English
• Subjects: active constituents; immunological activity; Liriodendron chinensis; 免疫活性; 成分; 抑制作用; 活性化合物; 生物活性测定; 脾淋巴细胞增殖; 腹腔巨噬细胞; 鹅掌楸属
• ISSN: 1674-6384; 2589-3610
• DOI: 10.1016/S1674-6384(15)60051-X

Objectives To investigate the anti-inflammatory components from the leaves of Liriodendron chinensis. Methods The 95% alcohol extract from the leaves of L. chinensis was subjected to column chromatography, and the structures of purified compounds were determined by spectral methods. The bioassay was performed through the inhibitory effects on the inflammatory cells activated by lipopolysaccharide （LPS）. Results Nine compounds were isolated, including octacosanoic acid （1）, stearic acid （2）, （2R-2-hydroxy-N-[（2S,3S,4R,8E- l ,3,4-tri-hydroxyicos-8-en- 2-yl]tetracosanamide （3）, （2 R）- 2- hyd roxy- N- [（2 S, 3 S,4 R,8 E）- 1 - O-β- D-glucopyranosyloxy-3,4-dihydroxy- octadec-8-en-2-yl]eicosanamide （4）, （2R）-2-hydroxy-N-[（2S,3S,4R,8E）-l-O-β-D- glucopyranosyloxy-3,4-dihydroxyoctadec-8-en-2-yl]hexadecanamide （5）, dicentrinone （6）, liriodenine （7）, daucosterol （8）, and liriodendritol （9） and among which five compounds could significantly lower the content of nitric oxide （NO） from peritoneal macrophages of rats induced by LPS and reduce the splenic lymphocyte proliferation in mice. This is the first report on the occurrence of ceramides and dicentrinone in the plants of Liriodendron Linn. Conclusion The five compounds are likely to be anti-inflammatory compounds concerning to their pronounced inhibitory action on the activated inflammatory cells. This assessment might provide a basis for searching the potent active compounds used for the treatment of inflammation.