Use of transgenic (knockout) mice reveals a site distinct from the α2A-adrenoceptors for agmatine in the vas deferens

The inhibitory effect of agmatine on electrically induced contractions was studied in vas deferens of Adra 2a transgenic mice lacking α2A-adrenoceptors. Agmatine and clonidine caused a concentration-dependent inhibition of twitches. However, while agmatine showed a similar pIC50 value in control and...

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Bibliographic Details
Published in:Pharmacological reports 2009-03, Vol.61 (2), p.325-329
Main Authors: Santos, Wilson C., Garcez-do-Carmo, Lucia, da Silva, Eliane C., Pascual, Ricardo de, Jurkiewicz, Neide H., Jurkiewicz, Aron, Gandía, Luis
Format: Article
Language:English
Subjects:
agmatine
Drug Safety and Pharmacovigilance
neurotransmission
Pharmacotherapy
Pharmacy
Short Communication
transgenic (knockout) mice
vas deferens
α2A-adrenoceptors
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Summary:The inhibitory effect of agmatine on electrically induced contractions was studied in vas deferens of Adra 2a transgenic mice lacking α2A-adrenoceptors. Agmatine and clonidine caused a concentration-dependent inhibition of twitches. However, while agmatine showed a similar pIC50 value in control and transgenic mice, the pIC50 value for clonidine was about 30-fold lower in knockout mice. In both strains, yohimbine shifted the curve for clonidine, but not for agmatine, even when a 100-fold higher concentration of yohimbine was employed. Our results indicate that inhibition by agmatine in mouse vas deferens is not simply due to interactions with α2-adrenoceptors in our experimental conditions.
ISSN:1734-1140
2299-5684
DOI:10.1016/S1734-1140(09)70039-8