Evaluation of chemopreventive effect of Fumaria indica against N-nitrosodiethylamine and CCl4-induced hepatocellular carcinoma in Wistar rats

• Published in: Asian Pacific journal of tropical medicine 2012-08, Vol.5 (8), p.623-629
• Main Authors: Hussain, Talib, Siddiqui, Hefazat H, Fareed, Sheeba, Vijayakumar, M, Rao, Chandana V
• Format: Article
• Language: English
• Subjects: Alkaline; Alkaline phosphatase; Animals; Antineoplastic Agents, Phytogenic - pharmacology; Antineoplastic Agents, Phytogenic - therapeutic use; Biomarkers, Tumor - metabolism; Body Weight - drug effects; Carbon Tetrachloride; carcinoma; Carcinoma, Hepatocellular - chemically induced; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - prevention & control; Diethylnitrosamine; Dose-Response Relationship, Drug; Drug Administration Schedule; Female; Fumaria; Fumaria indica; Hepatocellular; Hepatocellular carcinoma; indica; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver Neoplasms, Experimental - chemically induced; Liver Neoplasms, Experimental - metabolism; Liver Neoplasms, Experimental - prevention & control; Mice; N-nitrosodiethylamine; phosphatase; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; protein; Rats; Rats, Wistar; Treatment Outcome; α-feto; α-feto protein
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(12)60128-X

<正>Objective:To investigation the chemopreventive potential of Fumaria indica（F.indica） extract（FIE） on N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wislar rats.Methods:The experimental animals were divided into six groups（n=6）.Hepatocellular carcinoma was induced by single intraperitoneal injection of N-nitrosodiethylamine（NDEA） in normal saline at a dose of 200 mg/kg body weight followed by weekly subcutaneous injections of CCl4（3 mL/kg/week） for 6 weeks,as the promoter of carcinogenic effect.After administration of the carcinogen,200 and 400 mg/kg of FIE were administered orally once a day throughout the study.At the end of 20 weeks,the body weight,liver weight and relative liver weight were measured.The percentage of nodule incidence and liver cancer markers such as aspartate transaminase（AST）,alanine transaminase（ALT）,alkaline phosphatase（ALP）,γ-glutamyl transferase（γ-GT）,total bilirubin level（TBL）,α-feto protein（AFP） and carcinoembryonic antigen were estimated along with histopathological investigation in experimental groups of rats. Results:Obtained results demonstrated that the cotreatment with FIE significantly prevented the decrease of the body weight and also increased in relative liver weight caused by NDEA. The treatment with FIE significantly reduced the nodule incidence and nodule multiplicity in the rats after NDEA administration.The levels of liver cancer markers such as AST,ALT,ALP,γ-glutamyl transferase,TBL,AFP and carcinoembryonic antigen were substantially increased by NDEA treatment.However,FIE treatment significantly reduced the liver injury and restored the entire liver cancer markers.Histological observations of liver tissues too correlated with the biochemical observations.Conclusions:These finding powerfully supports that F.indica exert chemopreventive effect by suppressing the tumor burden and restoring the activities of hepatic cancer marker enzymes on NDEA and CCl4-induced hepatocarcinogenesis in Wistar rats.