AML-014 Expression Analysis, Clinical Significance, and Potential Function of ALOX5AP in Acute Myeloid Leukemia

• Published in: Clinical lymphoma, myeloma and leukemia myeloma and leukemia, 2024-09, Vol.24, p.S286-S287
• Main Authors: Goel, Harsh, Chopra, Anita, Ranjan, Amar, Meena, Jagdish Prasad, Gupta, Aditya Kumar, Viswanathan, Ganesh Kumar, Bakhshi, Sameer, Khan, Maroof Ahmad, Tanwar, Pranay
• Format: Article
• Language: English
• Subjects: ALOX5AP; AML; gene expression; methylation
• ISSN: 2152-2650
• DOI: 10.1016/S2152-2650(24)01144-3

Acute myeloid leukemia (AML) presents as a heterogeneous group of hematologic malignancies characterized by dysregulated proliferation and differentiation of leukemic primitive cells. Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) has been implicated in carcinogenesis, yet its role in AML remains under-studied. This work aimed to investigate the clinical and prognostic significance of the ALOX5AP gene in AML through analysis of its expression, methylation patterns, and molecular mechanisms. This work aimed to investigate the clinical and prognostic significance of the ALOX5AP gene in AML through analysis of its expression, methylation patterns, and molecular mechanisms. A total of 173 AML patients and 70 control cases were assessed for ALOX5AP gene expression and DNA methylation status. Kaplan–Meier survival estimation was employed to evaluate ALOX5AP's predictive importance. Correlations between ALOX5AP expression and functional states in AML single-cell datasets were estimated. Additionally, correlation analysis identified associated genes using the Linked Omics database, while gene set enrichment analysis (GSEA) elucidated molecular mechanisms of ALOX5AP in AML. ALOX5AP was significantly overexpressed and exhibited lower methylation levels in AML cohorts compared to controls (P