Cytoprotective effect of low-dose tacrolimus on islets of Langerhans in cultures subjected to stimulation by acute rejection cytokines

Abstract Introduction The improvement in pancreatic islet transplantation results is due to immunosuppression protocols that include, among others, low-dose tacrolimus. Both antiinflammatory and anti-oxidant effects of tacrolimus could be useful in preventing primary rejection. Aim To evaluate in vi...

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Bibliographic Details
Published in:Cirugia española (English ed.) 2010, Vol.87 (6), p.372-377
Main Authors: Balibrea del Castillo, José María, Arias-Díaz, Javier, García Martín, María Cruz, Vives-Pi, Marta, García Pérez, Juan Carlos, Cid, Ramón Cantero, Ameigeiras, Elena Vara, Balibrea Cantero, José Luis
Format: Article
Language:English
Subjects:
Apoptosis
Estrés oxidativo
Immunosuppression
Inmunosupresión
Oxidative stress
Pancreatic islet transplantation
Surgery
Tacrolimus
Trasplante de islotes pancreáticos
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Summary:Abstract Introduction The improvement in pancreatic islet transplantation results is due to immunosuppression protocols that include, among others, low-dose tacrolimus. Both antiinflammatory and anti-oxidant effects of tacrolimus could be useful in preventing primary rejection. Aim To evaluate in vitro islet low-dose tacrolimus response after pro-inflammatory stimulation. Material and methods Isolated rat islets were cultured in RPMI medium in the presence of IL-1 (50 UI/ml) plus IF-γ(1000 UI/ml) and tacrolimus (5 ng/ml). The 24 h production of lipoperoxide (LPO) and nitric oxide (NO) were measured as oxidative stress markers. Determination of apoptosis markers (nucleosome content and Bcl-2) was also performed. Results Oxidative stress (LPO 10.1 ± 1.16 pmol/isletx24; NO 19.1 ± 3.28 pmol/isletx24 h) and apoptosis (nucleosome 0.24 ± 0.04 UI/islet; Bcl-2 0.69 ± 0.212 UI/islet) markers showed a very significant increase after cytokine stimulation (P
ISSN:2173-5077
2173-5077
DOI:10.1016/S2173-5077(10)70184-8