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Isoperoxidase analysis on Thelypteris ciliata (Wall. ex Benth.) Holttum (Thelypteridaceae)

To assess the genetic variation between different populations of Thelypteris ciliata (Wall. ex Benth.) Holttum (T. ciliata) collected from different localities on Tirunelveli Hills (Kakachi, Kothayar Upper Dam) India, using isoperoxidase. The matured sterile and fertile fronds were harvested and ser...

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Bibliographic Details
Published in:Asian Pacific journal of tropical biomedicine 2012-01, Vol.2 (1), p.S27-S29
Main Authors: Johnson, M, Irudayaraj, V, Dominic, Rajkumar S
Format: Article
Language:English
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Summary:To assess the genetic variation between different populations of Thelypteris ciliata (Wall. ex Benth.) Holttum (T. ciliata) collected from different localities on Tirunelveli Hills (Kakachi, Kothayar Upper Dam) India, using isoperoxidase. The matured sterile and fertile fronds were harvested and served as explants for the isoperxidase analysis. The isoperoxidase isolation, separation and staining were followed by Smila et al. method. A total of six bands in six different positions with five active regions were observed in the isoperoxidase enzyme system of T. ciliata. The present study confirms the intra and inters-population's genetical difference of T. ciliata.
ISSN:2221-1691
2588-9222
DOI:10.1016/S2221-1691(12)60124-5