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Isoperoxidase analysis on Thelypteris ciliata (Wall. ex Benth.) Holttum (Thelypteridaceae)
To assess the genetic variation between different populations of Thelypteris ciliata (Wall. ex Benth.) Holttum (T. ciliata) collected from different localities on Tirunelveli Hills (Kakachi, Kothayar Upper Dam) India, using isoperoxidase. The matured sterile and fertile fronds were harvested and ser...
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Published in: | Asian Pacific journal of tropical biomedicine 2012-01, Vol.2 (1), p.S27-S29 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | To assess the genetic variation between different populations of Thelypteris ciliata (Wall. ex Benth.) Holttum (T. ciliata) collected from different localities on Tirunelveli Hills (Kakachi, Kothayar Upper Dam) India, using isoperoxidase.
The matured sterile and fertile fronds were harvested and served as explants for the isoperxidase analysis. The isoperoxidase isolation, separation and staining were followed by Smila et al. method.
A total of six bands in six different positions with five active regions were observed in the isoperoxidase enzyme system of T. ciliata.
The present study confirms the intra and inters-population's genetical difference of T. ciliata. |
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ISSN: | 2221-1691 2588-9222 |
DOI: | 10.1016/S2221-1691(12)60124-5 |