Quantitation of plasma total 15-series F2-isoprostanes by sequential solid phase and liquid–liquid extraction

F2-isoprostanes are stereo- and regioisomers of prostaglandin F2α (PGF2α) and are used as biomarkers for lipid peroxidation. We modified our liquid–liquid extraction (LLE) procedure for F2-isoprostane analysis (Anal. Biochem. 350 (2006) 41–51) to use a combination of solid phase extraction (SPE) and...

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Published in:Analytical biochemistry 2010-01, Vol.396 (2), p.319-321
Main Authors: Taylor, Alan W., Traber, Maret G.
Format: Article
Language:English
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Summary:F2-isoprostanes are stereo- and regioisomers of prostaglandin F2α (PGF2α) and are used as biomarkers for lipid peroxidation. We modified our liquid–liquid extraction (LLE) procedure for F2-isoprostane analysis (Anal. Biochem. 350 (2006) 41–51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC–tandem mass spectrometry (HPLC–MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF2α.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2009.09.042