The utility of stable isotope labeled (SIL) analogues in the bioanalysis of endogenous compounds by LC-MS applied to the study of bile acids in a metabolomics assay

The growing field of biomarker bioanalysis by liquid chromatography mass spectrometry (LC–MS) is challenged with the selection of suitable matrices to construct relevant and valid calibration curves resulting in not only precise but also accurate data. Because surrogate matrices are often employed w...

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Bibliographic Details
Published in:Analytical biochemistry 2016-06, Vol.503, p.71-78
Main Authors: Zheng, Joanna J., Shields, Eric E., Snow, Kimberly J., Nelson, David M., Olah, Timothy V., Reily, Michael D., Robertson, Donald G., Shipkova, Petia A., Stryker, Steven A., Xin, Baomin, Drexler, Dieter M.
Format: Article
Language:English
Subjects:
Animals
Bile acids
Bile Acids and Salts - blood
Bile Acids and Salts - chemistry
Bile Acids and Salts - metabolism
Chromatography, Liquid
Deuterated analogues
Humans
Isotope Labeling
LC–MS
Mass Spectrometry
Metabolomics
Molecular Structure
Naive matrix
Rats
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Summary:The growing field of biomarker bioanalysis by liquid chromatography mass spectrometry (LC–MS) is challenged with the selection of suitable matrices to construct relevant and valid calibration curves resulting in not only precise but also accurate data. Because surrogate matrices are often employed with the associated concerns about the accuracy of the obtained data, here we present an assay using surrogate analytes in naive biological matrices. This approach is illustrated with the analysis of endogenous bile acids (e-BAs) in serum and plasma using stable isotope-labeled (SIL) analogues as calibration standards to address the matrix concerns. Several deuterated BAs (d-BAs) were used as standards representing respectively grouped e-BAs with structural similarity allowing for the simultaneous bioanalysis of 16 e-BA. The utility of this LC–MS assay employing d-BAs is demonstrated with the analysis of samples resultant of a controlled metabolomics study where a cohort of rats was fed/fasted to investigate the change of e-BAs dependent on food consumption and fasting time.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2016.03.011