Flow analysis of p-aminophenyl phosphate with a gold nanoelectrode ensemble based detector

An alkaline phosphatase (AP) immobilized gold nanoelectrode ensemble (GNEE) was fabricated by electroless deposition of Au 3+ onto the nanopores ( d = 50 nm) of polycarbonate membranes and modifying the surface of GNEE successively with a self-assembled monolayer (SAM) of 2-aminoethanethiol, an alka...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2005-04, Vol.537 (1), p.315-319
Main Authors: Hsia, Tsung-Huang, Liao, Kuo-Tang, Huang, Hsuan-Jung
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Analytical chemistry
Chemistry
Electrochemical methods
Electroless deposition
Exact sciences and technology
Flow analysis
Gold nanoelectrode
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An alkaline phosphatase (AP) immobilized gold nanoelectrode ensemble (GNEE) was fabricated by electroless deposition of Au 3+ onto the nanopores ( d = 50 nm) of polycarbonate membranes and modifying the surface of GNEE successively with a self-assembled monolayer (SAM) of 2-aminoethanethiol, an alkaline phosphatase (AP) layer and a porous film of poly(4-vinylpyridine-co-styrene) (PVP). Performance of the AP–GNEE was studied by monitoring the current responses from the injection of p-aminophenyl phosphate ( p-APP) into a flow injection analysis (FIA) system. Optimal conditions such as concentration of AP for immobilization, pH of carrier solution, potential applied for the analysis of p-APP were studied. Calibration graph over a broad dynamic concentration range (0.1 μM to 1.0 mM) gave satisfactory linearity ( R 2 = 0.986) and a very low detection limit (0.1 μM) confirming the applicability and superiority of the AP–GNEE for p-APP analysis.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2005.01.023