Sandwich-type electrochemical aptasensor based on hollow mesoporous carbon spheres loaded with porous dendritic Pd@Pt nanoparticles as signal amplifier for ultrasensitive detection of cardiac troponin I

• Published in: Analytica chimica acta 2021-12, Vol.1188, p.339202, Article 339202
• Main Authors: Wang, Zhenxing, Zhao, Hongli, Chen, Kaicha, Li, Hongyuan, Lan, Minbo
• Format: Article
• Language: English
• Subjects: Aptamers, Nucleotide; Aptasensor; Biosensing Techniques; Carbon; Cardiac troponin I; Electrochemical Techniques; Hollow mesoporous carbon spheres; Humans; Hydrogen Peroxide; Limit of Detection; Metal Nanoparticles; Nanoparticles; Pd@Pt bimetallic nanoparticles; Porosity; Signal amplification; Troponin I
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2021.339202

Signal amplification is crucial to improve the sensitivity for the electrochemical detection of cardiac troponin I (cTnI), one of the ideal biomarkers for early acute myocardial infarction (AMI) diagnosis. Herein, we developed a novel signal amplification strategy to construct a sandwich-type electrochemical aptasensor for the detection of cTnI. Core-shell Pd@Pt dendritic bimetallic nanoparticles loaded on melamine modified hollow mesoporous carbon spheres (Pd@Pt DNs/NH2-HMCS) was prepared as labels to conjugate with thiol-modification DNA aptamers probe for signal amplification. While introducing numerous amino groups, the melamine functionalized hollow mesoporous carbon spheres (NH2-HMCS) retained the edge-plane-like defective sites for the adhesion and electrocatalytic reduction of H2O2. With the unique characteristics of NH2-HMCS, it not only enhanced the dispersity and loading capacity of core-shell Pd@Pt dendritic bimetallic nanoparticles (Pd@Pt DNs), but also improved the stability of bonding by the affinity interaction between Pd@Pt DNs and amino groups of melamine. Meanwhile, the synergistic catalysis effect between Pd@Pt DNs and NH2-HMCS significantly enhanced the electrocatalytic reduction of H2O2 and further amplified the signal. Under optimal conditions, this recommended aptasensor for cTnI detection displayed a wide dynamic range from 0.1 pg/mL to 100.0 ng/mL and a low detection limit of 15.4 fg/mL (S/N = 3). The sensor also successfully realized the analysis of cTnI-spiked human serum samples, meaning potential applications in AMI diagnosis. [Display omitted] •A facile strategy for preparing melamine-modified carbon materials as superb support matrix was presented.•Based on the strategy, amino groups-enriched NH2-HMCS retained its advantages of morphology characteristics.•Pd@Pt DNs/NH2-HMCS as a signal amplification label showed excellent electrocatalytic performance for the reduction of H2O2.•A novel sandwich-type electrochemical aptasensor was designed for detecting cTnI.•The sensitive aptasensor possessed a wide dynamic range and low detection limit for detecting cTnI.