Further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from Myracrodruon urundeuva seeds against Aedes aegypti

• Published in: Acta tropica 2015-12, Vol.152, p.49-55
• Main Authors: Souza, Terezinha M., Menezes, Erika S. Bezerra, Oliveira, Rodrigo V., Almeida Filho, Luiz Carlos P., Martins, Jorge M., Moreno, Frederico B., Monteiro-Moreira, Ana Cristina O., Moura, Arlindo A. Araripe, Carvalho, Ana F. Urano
• Format: Article
• Language: English
• Subjects: Aedes - drug effects; Anacardiaceae - chemistry; Animals; Comparative proteomics; Dengue - prevention & control; Detoxification; Insecticides - isolation & purification; Insecticides - pharmacology; Larva - drug effects; m-Pentadecadienyl-phenol; Natural insecticide; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Seeds - chemistry
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2015.08.010

[Display omitted] •The larvicidal activity of m-pentadecadienyl-phenol is based on the compound structure.•Proteomic analysis of larvae of Aedes aegypti after exposure to m-pentadecadienyl phenol showed 12 spots differentially expressed.•The natural insecticide caused elevated detoxification response.•The insecticide caused overexpression of proteins that could indicate generation of reactive oxygen species and liberation of lysosomal content.•Proteins overexpressed may lead to responses of apoptotic pathways. Nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector Aedes aegypti. Besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. Thus, the present work aimed to assess changes in protein expression of 3rd-instar larvae of Ae. aegypti after exposure to the natural insecticide m-pentadecadienyl-phenol. Bidimensional electrophoresis followed by mass spectrometry resulted in identification of 12 proteins differentially expressed between control and treated groups. Larvae exposed to the toxic compound for 24h showed elevated detoxification response (glutathione-S-transferase), increased levels of stress-related proteins (HSP70) as well as evidence of lysosome stabilization to enable survival. Furthermore, expression of proteins involved in protection of peritrophic membrane and metabolism of lipids indicated systemic effect of toxic effects in treated larvae.