Protein expression profile of Taenia crassiceps cysticerci related to Th1- and Th2-type responses in the mouse cysticercosis model

• Published in: Acta tropica 2020-12, Vol.212, p.105696, Article 105696
• Main Authors: Díaz-Zaragoza, Mariana, Jiménez, LucíaLucía, Hernández, Magdalena, Hernández-Ávila, Ricardo, Navarro, Luz, Ochoa-Sánchez, Alicia, Encarnación-Guevara, Sergio, Ostoa-Saloma, Pedro, Landa, Abraham
• Format: Article
• Language: English
• Subjects: 2DE; Animals; Biological processes; cysticerci; Cysticercosis - immunology; Cysticercosis - metabolism; Disease Models, Animal; Female; Helminth Proteins - analysis; MALDI-TOF MS; Mice; Mice, Inbred BALB C; Taenia crassiceps; Th1 Cells - immunology; Th1/Th2 response; Th2 Cells - immunology
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2020.105696

The intraperitoneal cysticercosis model with the Taenia crassiceps ORF strain in female BALB/cAnN mice has been widely used to study the immune response in cysticercosis. During early infection (2 weeks), the host develops a non-permissive Th1 response, whereas during late infection (8 weeks), molecules from the cysticerci induce a Th2 response that is permissive to parasite growth. The modulation of the Th2 response is induced by molecules excreted/secreted by the larval stage of the parasite. However, there is limited information regarding the response of cysticerci to the mouse immunological environment during infection. The proteomic profiles in T. crassiceps ORF cysticerci when faced with the mouse Th1 and Th2 responses were analyzed through two-dimensional gel electrophoresis (2DE), and the differential expression of proteins was evaluated. Thirteen proteins, whose differential expression varied between 70% and 100%, were selected randomly. Protein identification by MALDI-TOF MS and BLAST showed that the proteins were related to folding, signaling, enzymatic activities, cell-movement regulation, cell-cell interactions, motility, carbohydrate metabolism, detoxification, and redox regulation processes. Notably, some of the proteins can act as antigenic-protective molecules and elicit a weak Th1 response; however, most are involved in the avoidance of the immune system, which leads to a Th2 response, or apoptosis. The findings indicate the process by which T. crassiceps cysticerci responds based on the host environment and provides novel insights into the mechanism by which this facilitates its establishment and persistence in the mouse. Furthermore, these proteins could be used as targets for drug and vaccine development.