Novel nanostructured biodegradable polymer matrices fabricated by phase separation techniques for tissue regeneration

Biomimetic nanostructures have a wide range of applications. In particular, biodegradable polymer nanostructures that mimic the subtleties of extracellular matrix may provide favorable cell interactions. In this study, a co-solvent system was developed to configure a thermodynamically metastable bio...

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Bibliographic Details
Published in:Acta biomaterialia 2013-06, Vol.9 (6), p.6915-6927
Main Authors: Hsu, S.-h., Huang, S., Wang, Y.-C., Kuo, Y.-C.
Format: Article
Language:English
Subjects:
3T3 Cells
Absorbable Implants
Animals
biodegradability
Biodegradable
Biomimetic Materials - chemical synthesis
biomimetics
cell culture
chitosan
coatings
Equipment Design
Equipment Failure Analysis
extracellular matrix
Extracellular Matrix - chemistry
gene expression
genetic markers
glucose
Guided Tissue Regeneration - instrumentation
humans
Materials Testing
Matrices
Mice
mineralization
nanofibers
Nanofibers - chemistry
Nanofibers - ultrastructure
Nanostructure
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - physiology
Osteoinduction
Phase separation
Phase Transition
Polymers - chemistry
polyurethanes
stem cells
thermodynamics
tissue repair
Tissue Scaffolds
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Summary:Biomimetic nanostructures have a wide range of applications. In particular, biodegradable polymer nanostructures that mimic the subtleties of extracellular matrix may provide favorable cell interactions. In this study, a co-solvent system was developed to configure a thermodynamically metastable biodegradable polymer solution, from which novel nanostructured matrices subsequently formed via wet phase separation (quaternary) or a combination with thermally induced phase separation. Three-dimensional (3D) nanostructured porous matrices were further fabricated by combination with particle-leaching (100–300μm glucose). The new co-solvent system may generate matrices with reproducible nanostructures from a variety of biodegradable polymers such as poly(d,l-lactide) (PLA), poly(ε-caprolactone) (PCL) and PCL-based polyurethane. In vitro cell culture experiments were performed with mouse pre-osteoblasts (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hBM-MSC) to evaluate the osteoinductive potential of PLA nanostructures. The results showed that nanofibrous (
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2013.02.012