Effects of different irrigation methods on nitrous oxide emissions and ammonia oxidizers microorganisms in greenhouse tomato fields

• Published in: Agricultural water management 2018-04, Vol.203, p.115-123
• Main Authors: Ye, X.H., Han, B., Li, W., Zhang, X.C., Zhang, Y.L., Lin, X.G., Zou, H.T.
• Format: Article
• Language: English
• Subjects: amoA gene; DNA-SIP; Greenhouse; qPCR; Soil N2O emissions
• ISSN: 0378-3774; 1873-2283
• DOI: 10.1016/j.agwat.2018.03.012

•Irrigation methods affects the soil N2O emissions in greenhouse field.•The DNA-SIP technique is used to determine microbial activity of ammonia oxidizing archaea (AOA) and bacteria (AOB) amoA gene.•Relationships between soil N2O flux and soil properties, gene abundance are established. Agricultural soils are strong sources of the potent greenhouse gas N2O but soil N2O emissions and its microbial mechanism in greenhouse field, especially ammonia-oxidizing microorganisms are unclear. We characterized a potential response in soil N2O production and its influencing factors, such as soil temperature, moisture, pH, and inorganic nitrogen to different irrigation methods named drip irrigation (DI), subsurface irrigation (SI) and furrow irrigation (FI) in a long-term irrigation field in greenhouse. The abundance and metabolic activity of ammonia oxidizing archaea (AOA) and bacteria (AOB) in greenhouse soils were also investigated using amoA gene as a molecular biomarker by quantitative PCR and 13CO2-DNA-stable isotope probing (SIP) methods. Results showed that N2O flux peaks would obviously occur within 1–8 days after each irrigation. The soil N2O flux in FI treatment was significantly higher than that in DI and SI treatments (P