Synergistic therapeutic effect of nano-honokiol and 5-fluorouracil on the induced-tongue cancer in rats

This research aims to investigate the effect of combining 5-FU with honokiol-nanoparticles (HNK-NP) on enhancing the anti-cancerous activity of 5-FU without intensifying its toxicity to normal tissues. Honokiol (HNK) was formulated in nano-particles (HNK-NPs) for better bioavailability, efficient pe...

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Bibliographic Details
Published in:Journal of oral and maxillofacial surgery, medicine, and pathology medicine, and pathology, 2020-11, Vol.32 (6), p.556-562
Main Authors: Swidan, Sara A, Hassan, Magda MA, Elmansy, Merhan N, Swidan, Shady A
Format: Article
Language:English
Subjects:
4-Nitroquinoline oxide
Fluorouracil
Honokiol
Nanoparticles
Tongue carcinoma
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Summary:This research aims to investigate the effect of combining 5-FU with honokiol-nanoparticles (HNK-NP) on enhancing the anti-cancerous activity of 5-FU without intensifying its toxicity to normal tissues. Honokiol (HNK) was formulated in nano-particles (HNK-NPs) for better bioavailability, efficient penetration, and sustained release. HNK-NPs were successfully prepared using the nano-precipitation technique and evaluated using the transmission electron microscope (TEM). It was used in combination with 5-FU for treatment of tongue carcinoma-induced chemically by 4-nitroquinoline oxide (4NQO) in Wistar albino rats. They were divided into five groups (10 animals each) including untreated control, 4NQO, 5-FU, HNK-NPs, and 5-FU with HNK-NPs. Assessment of liver enzymes and creatinine was done to evaluate the safety of the used drugs. Serial sections of the tongues were examined histologically, immunohistochemical (IHC) detection of the P53 protein, and real-time PCR (polymerase chain reaction) of P53 gene expression were also assessed. The results showed that treatment with both 5-FU + HNK-NPs had significantly regressed tumors induced by the carcinogen, with a marked reduction in the systemic toxicity as compared with either drug individually. Data analysis showed statistically significant differences (P < 0.05) in the expression levels of P53 either through IHC or molecular detection between the combined treatment and positive control groups. The combination therapy including 5-FU and HNK-NPs has substantial potential as an anti-cancer chemotherapeutic strategy.
ISSN:2212-5558
2212-5566
DOI:10.1016/j.ajoms.2020.06.003