Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures

Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this s...

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Bibliographic Details
Published in:Algal research (Amsterdam) 2018-07, Vol.33, p.16-27
Main Authors: Xing, GuanLan, Yuan, HongLi, Yang, JinShui, Li, JinYu, Gao, QuanXiu, Li, WeiLin, Wang, EnTao
Format: Article
Language:English
Subjects:
Auxenochlorella protothecoides
Fatty acid metabolism
iTRAQ-based proteomics
Lipid accumulation
Temperature stress
Transcriptomics
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Summary:Temperature is one of the critical environmental factors that influence microalgal growth, lipid content and fatty acid (FA) composition. However, the molecular mechanism underlying regulations of FA metabolism under low and high temperature stress in oleaginous microalgae remains unclear. In this study, integrated analyses of transcriptome, proteome and fatty acid profilings were performed for the first time in Auxenochlorella protothecoides UTEX 2341. Under low and high temperature (LT and HT) stress, a total of 5565 and 4757 genes, and 1311 and 728 proteins were differentially expressed respectively. 65 actively expressed genes and 61 proteins involved in FA metabolism were identified. A strong positive correlation between the genes' transcript and protein levels existed in FA metabolism (r = 0.80, p-value 
ISSN:2211-9264
2211-9264
DOI:10.1016/j.algal.2018.04.028