Enzymatic synthesis and characterization of chlorophyllide derivatives as possible internal standards for pigment chromatographic analysis

•Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll substrates.•Chromatographic properties of esters of different alcohols and diols were studied.•Chlorophyllide a (8′-hydroxyoctyl) ester was selected and com...

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Bibliographic Details
Published in:Algal research (Amsterdam) 2020-03, Vol.46, p.101688, Article 101688
Main Authors: Gavalás-Olea, Antonio, Sanz, Noelia, Riobó, Pilar, Garrido, José Luis, Vaz, Belén
Format: Article
Language:English
Subjects:
Chlorophyllide sters
Chromatography
Internal Standard
Pigment analysis
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Summary:•Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll substrates.•Chromatographic properties of esters of different alcohols and diols were studied.•Chlorophyllide a (8′-hydroxyoctyl) ester was selected and completely characterized.•The proposed internal standard showed adequate stability over time. In this article the chlorophyllase activity of Dunaliella salina has been employed to generate different chlorophyllide a and b esters that could potentially be used as internal standards in pigment analysis. Chlorophyllide a (8’-hydroxyoctyl) ester was selected due its adequate chromatographic and spectral properties and was fully characterized by UV-Vis, ESI-MS and NMR. An easy room temperature procedure for its synthesis is described. Attachment
ISSN:2211-9264
2211-9264
DOI:10.1016/j.algal.2019.101688