Ultrasound pretreatment to enhance the enzymatic hydrolysis of Dictyota dichotoma for sugars production

• Published in: Algal research (Amsterdam) 2023-04, Vol.71, p.103083, Article 103083
• Main Authors: Romero-Vargas, Agustín, Muñoz, Inmaculada, Marzo, Cristina, Díaz, Ana B., Romero-García, Luis I., Blandino, Ana
• Format: Article
• Language: English
• Subjects: Brown macroalgae; Dictyota dichotoma; Enzymatic hydrolysis; Reducing sugars; Ultrasound pretreatment
• ISSN: 2211-9264; 2211-9264
• DOI: 10.1016/j.algal.2023.103083

There is an increasing demand for natural resources for biorefining purposes. In this regard, the efficient use of seaweed seems promising as they represent a sustainable feedstock with advantageous characteristics, given their abundant concentration of carbohydrates and low lignin levels. However, in the context of sugar platform biorefineries, to make this activity sustainable, high availability of seaweed biomass is required. In this respect, beach-cast seaweed biomass represents an attractive alternative, reducing the negative impact associated with its accumulation and decomposition on the beaches, which affects the financial income of the region. In this study, Dictyota dichotoma deposited on the coasts of Cadiz (Spain) was collected at the end of the autumn season and used as raw material for the production of simple sugars in the frame of a biorefinery approach. The potential of ultrasound pretreatment was applied to two biomass concentrations (2.1 and 4.3 % w/v), at different amplitude percentages (20, 40 and 60 %) and pretreatment times (15, 40 and 60 min) to enhance algal biomass saccharification. The best condition for reducing sugars (RS) production was: 4.3 % w/v of algal suspension and 40 min pretreatment at 40 % amplitude. This condition supposes specific energy (Es) of 6.78 MJ/kg and leads to a total sugar concentration of 163.64 ± 0.93 mg RS/g dried biomass. A slight improvement in RS production could be attained by increasing the amplitude to 60 % (165.52 ± 3.89 mg/g) but requiring 50 % higher specific energy. Experimental data were fitted to simple first-order kinetics. The hydrolysis rate constant k was a function of the biomass concentration and practically independent of the energy applied. In addition, the average value of k was 8.6 times higher than that for non-pretreated seaweed. [Display omitted] •Beach-cast seaweed are used to obtain reducing sugars (up to 164–166 mg/g biomass).•Ultrasound pretreatment improves the subsequent enzymatic hydrolysis of seaweed.•Optimum pretreatment conditions are 40 % amplitude and 40 min of sonication time.•Lower specific energy is required than in other physical-mechanical pretreatments.•The hydrolysis constant rate is 8.6 times higher than for non-pretreated biomass.