A comparative study of metabolites profiles, anti-inflammatory and antioxidant activity of methanolic extracts from three Arthrospira strains in RAW 264.7 macrophages

Arthrospira (spirulina), is a cyanobacterium endowed with antioxidant and anti-inflammatory activity, but strain-dependent production of bioactive compounds may impact these properties. In LPS-stimulated RAW 264.7 macrophages, we compared the antioxidant and anti-inflammatory activity of methanolic...

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Published in:Algal research (Amsterdam) 2023-06, Vol.73, p.103171, Article 103171
Main Authors: Bigagli, Elisabetta, D'Ambrosio, Mario, Cinci, Lorenzo, Pieraccini, Giuseppe, Romoli, Riccardo, Biondi, Natascia, Niccolai, Alberto, Rodolfi, Liliana, Tredici, Mario R., Luceri, Cristina
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Language:English
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Arthrospira
Carotenoids
Glycolipids
Inflammation
Microalgae
Spirulina
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creator Bigagli, Elisabetta
D'Ambrosio, Mario
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Tredici, Mario R.
Luceri, Cristina
description Arthrospira (spirulina), is a cyanobacterium endowed with antioxidant and anti-inflammatory activity, but strain-dependent production of bioactive compounds may impact these properties. In LPS-stimulated RAW 264.7 macrophages, we compared the antioxidant and anti-inflammatory activity of methanolic extracts from two A. platensis (F&M-C256 and F&M-C260), and an A. maxima (F&M-C258) strains. F&M-C260 (5–25 μg/mL) concentration dependently inhibited LPS-induced NO release (p 
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Metabolites profiles, explored by HPLC-UV and HPLC-HRMS, revealed that F&M-C260 and F&M-C256 were the richest in β-cryptoxanthin and monogalactosyl monoglycerides respectively, with minor differences in zeaxanthin and β-carotene content. 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Metabolites profiles, explored by HPLC-UV and HPLC-HRMS, revealed that F&M-C260 and F&M-C256 were the richest in β-cryptoxanthin and monogalactosyl monoglycerides respectively, with minor differences in zeaxanthin and β-carotene content. These results suggest that the antioxidant activity of the extracts was due to a direct reducing effect rather than to the transcriptional activation of SOD2 and HO-1 and that their differential and multitarget anti-inflammatory effects were correlated to differences in metabolites profiles. •Arthrospira strains differ in carotenoids and monogalactosyl mono-glycerides content.•Arthrospira strains have a multitarget anti-inflammatory activity.•A. platensis strains mainly inhibit iNOS-COX2/PGE2 pathway.•Maxima strain mainly inhibit NLRP3/ IL1-β pathway.]]></abstract><pub>Elsevier B.V</pub><doi>10.1016/j.algal.2023.103171</doi><oa>free_for_read</oa></addata></record>
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subjects Arthrospira
Carotenoids
Glycolipids
Inflammation
Microalgae
Spirulina
title A comparative study of metabolites profiles, anti-inflammatory and antioxidant activity of methanolic extracts from three Arthrospira strains in RAW 264.7 macrophages
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