Efficacy of novel 6-phytase from Buttiauxella sp. on ileal and total tract nutrient digestibility in growing pigs fed a corn-soy based diet

• Published in: Animal feed science and technology 2015-12, Vol.210, p.217-224
• Main Authors: Velayudhan, D.E., Heo, J.M., Dersjant-Li, Y., Owusu-Asiedu, A., Nyachoti, C.M.
• Format: Article
• Language: English
• Subjects: Amino acids; Calcium; Digestibility; Phosphorus; Phytase; Pig
• ISSN: 0377-8401; 1873-2216
• DOI: 10.1016/j.anifeedsci.2015.10.005

•We determined the efficiency of a Buttiauxella 6-phytase on nutrient digestibility in growing pigs.•The results showed a linear or quadratic response on most of the AA digestibility.•Ileal and total tract digestibility of Ca, P and other nutrients enhanced in a dose dependent manner. The current study evaluated the effect of different levels of a 6-phytase from Buttiauxella sp. expressed in Trichoderma reesei on ileal and total tract nutrient digestibility in growing pigs. Twelve ileal cannulated pigs (initial BW=25kg) were randomly assigned to 1 of 6 treatments in a replicated 6×6 Latin square design to give 12 observations per treatment. The experimental diets consisted of a corn soybean meal-based control diet (NC), NC supplemented with 4 levels of phytase (i.e. 250, 500, 1000 and 2000FTU/kg) and a low-protein diet (5% casein) used to quantify endogenous amino acid (AA) losses. All diets contained titanium dioxide as an indigestible marker. Pigs were given their daily feed allowance at a rate of 45g/kg BW determined at the beginning of each experimental period. Each period lasted for 9d with 5d of adaptation to experimental diets followed by 2d of faecal and 2d of ileal digesta collection. Data were analyzed using the mixed model procedures of SAS. The final model had treatment as the main effect as pen and period effects were non-significant. Increasing levels of 6-phytase supplementation linearly increased (P