Fertility disturbances of dimethylacetamide and glycerol in rooster sperm diluents: Discrimination among effects produced pre and post freezing-thawing process

With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results i...

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Bibliographic Details
Published in:Animal reproduction science 2017-09, Vol.184, p.228-234
Main Authors: Abouelezz, F.M.K., Sayed, M.A.M., Santiago-Moreno, J.
Format: Article
Language:English
Subjects:
Acetamides - pharmacology
Animals
Avian sperm
Chickens - physiology
Cryodamage
Cryopreservation
Cryoprotective Agents - pharmacology
DMA
Fertility - drug effects
Freezing
Glycerol
Glycerol - pharmacology
Male
Sperm-egg penetration
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Summary:With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results in a small portion of thawed live sperm with lesser fertilizing ability compared with the semen samples immediately after collection. This study was conducted to assess the pre-freezing damage occurring to the sperm due to the interaction with the cryoprotectants (CPAs) GLY (8%) and DMA (5%), as well as the post-freezing damage resulting from both freezing methods Data for each treatment, in fresh and frozen-thawed samples, were compared for sperm motility, fertilizing capacity and sperm-egg penetration holes/germinal disc (SP holes/GD). Hens (n=50) were artificially inseminated (10 hens/treatment) six times with 3day intervals between inseminations. The treatment of fresh sperm with DMA led to a reduction (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2017.07.021