The in vitro effect of ZnO nanoparticles on stallion spermatozoa quality

Nanoparticles have found their use mainly in medicine, where they are used as drug carriers because their dimensions allow them to circulate throughout the body and enter directly into the cell. The aim of this study was to determine the effect of ZnO nanoparticles dissolved in physiological solutio...

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Bibliographic Details
Published in:Animal reproduction science 2022-12, Vol.247, p.107129, Article 107129
Main Authors: Halo, Marko, Tirpák, Filip, Massányi, Martin, Dianová, Lucia, Massányi, Peter
Format: Article
Language:English
Subjects:
CASA
in vitro
MTT
spermatozoa
stallion
ZnO nanoparticles
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Summary:Nanoparticles have found their use mainly in medicine, where they are used as drug carriers because their dimensions allow them to circulate throughout the body and enter directly into the cell. The aim of this study was to determine the effect of ZnO nanoparticles dissolved in physiological solution (A: 0.75; B: 1.5; C: 3; D: 6; E: 12 and F: 24mg/mL) on stallion spermatozoa. Control samples (CON) were prepared by diluting ejaculate only with the physiological solution. Using the CASA system (Computer Assisted Semen Analysis), the individual motility parameters were analysed at 5 °C at different time intervals (0, 30, 60 and 90min). Stallion sperm viability was analysed using the mitochondrial toxicity test (MTT). Descriptive statistical characteristics (mean, standard deviation) together with One-way ANOVA and Dunnett’s post-test was selected for statistical evaluations (GraphPad 8 software). After 30min of culture, a lower percentage of motile spermatozoa was recorded only in samples E (p
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2022.107129