Taurine added to cryoconservation extender mitigates the cryodamage of bull spermatozoa

Artificial insemination is a necessary biotechnological tool widely used in cattle breeding with the aim to intensify milk and meat production. Thus, it is a frequently researched topic to minimize the cryodamage by the addition of natural and synthetic substances with antioxidant properties. Taurin...

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Bibliographic Details
Published in:Animal reproduction science 2022-12, Vol.247, p.107141, Article 107141
Main Authors: Tirpák, Filip, Halo, Marko, Dianová, Lucia, Slanina, Tomáš, Tirpák, Ivan, Massányi, Peter
Format: Article
Language:English
Subjects:
artificial insemination
bovine
cryopreservation
spermatozoa
taurine
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Summary:Artificial insemination is a necessary biotechnological tool widely used in cattle breeding with the aim to intensify milk and meat production. Thus, it is a frequently researched topic to minimize the cryodamage by the addition of natural and synthetic substances with antioxidant properties. Taurine is an amino acid synthetised from cysteine and methionine and is well known for its cytoprotective and antioxidant features. The aim of the present study was to evaluate the effect of taurine on bull spermatozoa during cryoconservation. Semen from five bulls was processed according to routine freezing protocols. Contrary to the control group (C), the experimental group T was enriched with the taurine dissolved in saline (200mM). After 1 mo of storage in liquid nitrogen, control and experimental insemination doses were thawed and analyzed. CASA analysis was performed 30, 60, and 90min post-thawing with results indicating enhanced total and progressive motility (p
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2022.107141