An NIR-II light-responsive nanoplatform for photothermal and enhanced photodynamic synergistic therapy toward drug-resistant bacterial infection and hypoxia relief in subcutaneous wound healing

• Published in: Applied materials today 2023-06, Vol.32, p.101839, Article 101839
• Main Authors: Meng, Wei, Zhang, Lian-Ying, Chen, Xiao-Tong, Ge, Feng-Jun, Huang, Peng, Chen, Yong-Jian, Huang, Ting, Huang, Nai-Han, Zou, Yi-Ming, Zhang, Wen-Hua, Zhang, Qun, Chen, Jin-Xiang
• Format: Article
• Language: English
• Subjects: Light-enhanced catalytic activity; NIR-II; Palladium nanoshell; Subcutaneous wound healing; Synergistic antibacterial therapy
• ISSN: 2352-9407; 2352-9415
• DOI: 10.1016/j.apmt.2023.101839

•A NIR-II light-responsive nanoplatform UiO-66-NH2@Pdshell-IR780 (UPI) was successfully constructed.•UiO-66-NH2@Pdshell has ideal photothermal performance and excellent photothermal conversion efficiency (44.9%).•UiO-66-NH2@Pdshell retains the catalytic activity of palladium nanoshells and has light-enhanced catalase-like activity to generate O2 to relief the hypoxic microenvironment and facilitate photodynamic therapy of IR780.•UPI can relieve inflammation and accelerate the healing of subcutaneous wounds through rapid removal of biofilm of MRSA and continuous oxygen supply strategy based on regulation microenvironment. The drug-resistant bacterial infection has seriously threatened human health and thus it is urgent to develop new and effective drugs to overcome the antibiotic resistance crisis. Herein, a near-infrared-II (NIR-II) region light-responsive Pd rough nanoshells on metal-organic framework (MOF) of UiO-66-NH2 (UiO-66-NH2@Pdshell) has been developed for the first time, which was further loaded with the photosensitizer IR780 to ultimately yield UiO-66-NH2@Pdshell-IR780 (UPI) as the core-shell theranostic nanoplatform. The Pd rough nanoshells demonstrate promising photothermal conversion efficiency, superoxide dismutase (SOD)-like activity, and light-enhanced catalase (CAT)-like activity. In the non-healing wound inflammatory microenvironment with overexpression of reactive oxygen species (ROS) caused by subcutaneous bacterial infection, Pd rough nanoshells in UPI transforms ROS, such as O2•−, into H2O2 and efficiently catalyzes the decomposition of the formed and endogenous H2O2 to produce O2. The O2 production process could be enhanced by NIR-II light to facilitate photodynamic efficacy of IR780 to prime rapid clearance of methicillin-resistant Staphylococcus aureus (MRSA) through biofilm inhibition, along with down-regulation of hypoxia inducible factor-1α (HIF-1α) and relief of inflammation, thus promote wound healing. Moreover, UPI exhibits negligible biotoxicity in vitro and in vivo. This work provides a method for preparing promising Pd-based nanomaterials toward subcutaneous infection treatment and wound healing. [Display omitted]