Evaluating the radioprotective effect of Cimetidine, IMOD, and hybrid radioprotectors agents: An in-vitro study

There are various radioprotective agents with different mechanisms that help to decrease ionizing radiation side effects. The radioprotective effect of Cimetidine and IMOD was assessed individually and compared with the hybrid radioprotectors agents (HRPAs-IMOD and Cimetidine) on human lymphocyte ce...

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Bibliographic Details
Published in:Applied radiation and isotopes 2021-08, Vol.174, p.109760, Article 109760
Main Authors: Rahgoshai, Siroos, Mehnati, Parinaz, Aghamiri, Mahmoud Reza, Haghighi Borujeini, Meysam, Banaei, Amin, Tarighatnia, Ali, Nader, Nader D., Kiapour, Mohammad, Abedi-Firouzjah, Razzagh
Format: Article
Language:English
Subjects:
Case-Control Studies
Cells, Cultured
Cimetidine
Cimetidine - administration & dosage
Cimetidine - pharmacology
Electron Spin Resonance Spectroscopy
Humans
Hybrid radio-protector agents
Immunologic Factors - administration & dosage
Immunologic Factors - pharmacology
IMOD
In Vitro Techniques
Lymphocytes
Micronucleus assay
Micronucleus Tests - methods
Radiation protection
Radiation-Protective Agents - administration & dosage
Radiation-Protective Agents - pharmacology
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Summary:There are various radioprotective agents with different mechanisms that help to decrease ionizing radiation side effects. The radioprotective effect of Cimetidine and IMOD was assessed individually and compared with the hybrid radioprotectors agents (HRPAs-IMOD and Cimetidine) on human lymphocyte cells. Twenty healthy volunteers (ten men and ten women) participated in the present study. About 75 mL peripheral blood lymphocytes from each individual were collected, and they were divided into 36 groups. Briefly, the blood samples were treated with different concentrations of Cimetidine (12.6 and 25.2 μg/mL) and IMOD (0.04, 0.08, and 0.12 mg/mL), and also a combination of these agents, namely hybrid radioprotectors agents (HRPAs). Besides, the irradiated groups were exposed to 2 and 4 Gy of Co-60 gamma irradiation. The amount of cellular damage was assessed using the micronucleus assay. The repeated measurements and paired T-test statistical analysis were used to compare the micronucleus frequencies in different groups. The micronucleus frequencies were significantly reduced (p 
ISSN:0969-8043
1872-9800
DOI:10.1016/j.apradiso.2021.109760