Comparison of plate count, microscopy, and DNA quantification methods to quantify a biocontrol fungus

•Quantitative PCR (qPCR) was evaluated as a quantification tool for a biocontrol fungus.•DNA estimates did not always correspond to either recoverable population or hyphal biomass.•However, DNA estimates increased as either recoverable population or biomass increased.•qPCR can reflect relative chang...

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Bibliographic Details
Published in:Applied soil ecology : a section of Agriculture, ecosystems & environment ecosystems & environment, 2016-02, Vol.98, p.285-288
Main Authors: Kim, Tae Gwan, Knudsen, Guy R.
Format: Article
Language:English
Subjects:
biological control
biomass
DNA
fungi
hyphae
image analysis
microscopy
pellets
plate count
Quantitative PCR
quantitative polymerase chain reaction
soil ecology
temporal variation
Trichoderma
Trichoderma harzianum
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Summary:•Quantitative PCR (qPCR) was evaluated as a quantification tool for a biocontrol fungus.•DNA estimates did not always correspond to either recoverable population or hyphal biomass.•However, DNA estimates increased as either recoverable population or biomass increased.•qPCR can reflect relative changes of active and inactive fungal propagules. DNA quantification has become a conventional method for quantifying filamentous fungi in environments. In this study, quantitative PCR was evaluated as a quantification tool by comparisons with plate count (unit, recoverable population) and microscopy with image analysis (hyphal biomass). The genetic transformant Trichoderma harzianumThzID1-M3 was used as a model organism. A soil microcosm experiment with different numbers of ThzID1-M3 alginate pellets showed that DNA was significantly correlated with biomass (P
ISSN:0929-1393
1873-0272
DOI:10.1016/j.apsoil.2015.10.010