Diminished rhizosphere and bulk soil microbial abundance and diversity across succession stages in Karst area, southwest China

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment ecosystems & environment, 2021-02, Vol.158, p.103799, Article 103799
• Main Authors: Xu, Huifang, Du, Hu, Zeng, Fuping, Song, Tongqing, Peng, Wanxia
• Format: Article
• Language: English
• Subjects: Forest soil; Karst area of China; Microbe community structure; Rhizosphere effect; Secondary succession
• ISSN: 0929-1393; 1873-0272
• DOI: 10.1016/j.apsoil.2020.103799

Soil microorganisms are important for plant nutrients and health. However, our understanding of soil microbial communities in the rhziosphere and bulk soils as plant succession stages increased in the Karst area remains unclear. Here, we evaluated how the bacterial and fungal communities of rhizosphere and bulk soils respond to different vegetation succession stages: grassland (GR), shrubland (SH), secondary forest (SF), and primary forest (PF). The abundance and composition of bacteria and fungi were determined by qPCR and MiSeq high-throughput sequencing methods, respectively. The results showed that the abundance and diversity in rhizosphere and bulk soils were diminished among vegetation succession stages. We found that SOC led to high microbial abundance in GR and SH during the period of primary succession stages, while AP potentially led to low abundance of microbes in the PF and SF of subsequent succession stages. Additionally, the OTUs shared by four succession stages (common OTUs) comprised approximately 87.61 ‐ 98.90% of the total relative abundance of bacteria and fungi in the rhizosphere and bulk soils, which indicated that the composition of fungi and bacteria in these environments were similar across different succession stages. pH might be an important driving factor involved in the similarity of bacterial and fungal compositions among different succession stages. However, the relative abundance of bacterial and fungal phyla across the four vegetation stages was different, and also determined by multiple soil properties. pH was the main factor determining bacterial community structure in rhizosphere soils, whereas SOC was the principal determinant of the same parameter for bacteria in bulk soils, and in both the rhizosphere and bulk soils for fungi. Overall, our results suggested that bacterial and fungal communities in the rhizosphere and bulk soils diminished with vegetation stages, which may provide evidence that mechanisms underlying the soil microbe-driven vegetation succession in the Karst area of southwest China. •The soil properties, other than the plant types and root effect, induced the changes of abundance and composition of bacteria and fungi in rhizosphere and bulk soils.•The SOC induced significant changes in the abundance of bacteria and fungi in the GR and SH in both rhizosphere and bulk soils of the primary succession stages. AP potentially lead to low abundance of microbes in the PF and SF in both rhizosphere and bulk soils