Atomic level analysis of biomolecules by the scanning atom probe

• Published in: Applied surface science 2009-11, Vol.256 (4), p.1210-1213
• Main Authors: Nishikawa, Osamu, Taniguchi, Masahiro, Ikai, Atsushi
• Format: Article
• Language: English
• Subjects: Atomic and molecular physics; Atomic masses, mass spectra, abundances and isotopes; Atomic properties and interactions with photons; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Exact sciences and technology; Field evaporation; Fragment ions; Leucine; Methionine; Physics; Properties of atoms and atomic ions; Scanning atom probe; Single walled carbon nanotubes
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2009.05.154

Utilizing the unique features of the scanning atom probe (SAP) the binding states of the biomolecules, leucine and methionine, are investigated at atomic level. The molecules are mass analyzed by detecting a single atom and/or clustering atoms field evaporated from a specimen surface. Since the field evaporation is a static process, the evaporated clustering atoms are closely related with the binding between atoms forming the molecules. For example, many thiophene radicals are detected when polythiophene is mass analyzed by the SAP. In the present study the specimens are prepared by immersing a micro cotton ball of single walled carbon nanotubes (SWCNT) in the leucine or methionine solution. The mass spectra obtained by analyzing the cotton balls exhibit singly and doubly ionized carbon ions of SWCNT and the characteristic fragments of the molecules, CH 3, CHCH 3, C 4H 7, CHNH 2 and COOH for leucine and CH 3, SCH 3, C 2H 4, C 4H 7, CHNH 2 and COOH for methionine.