Carbon nanotube-coating accelerated cell adhesion and proliferation on poly (L-lactide)

• Published in: Applied surface science 2012-12, Vol.262, p.24-27
• Main Authors: Hirata, Eri, Akasaka, Tsukasa, Uo, Motohiro, Takita, Hiroko, Watari, Fumio, Yokoyama, Atsuro
• Format: Article
• Language: English
• Subjects: Carbon nanotubes; Cell adhesion; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Osteoblast like cell; Physics; Poly(l-lactide)
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2012.01.012

► The surface of a polylactic acid (PLLA) was coated multiwalled carbon nanotubes (MWCNTs). ► MWCNT-coated PLLA showed remarkable higher wettability than uncoated PLLA. ► More Human osteosarcoma cell line (Saos2) adhered on the CNT-coated than those on uncoated PLLA at 2h after seeding. ► MWCNT-coating on PLLA improved the surface wettability and initial cell attachment at early stage. The surface of a polylactic acid (PLLA) was coated multiwalled carbon nanotubes (MWCNTs) in order to improve the surface properties. In addition, its surface characteristics and cell culturing properties were examined. Whole surface of PLLA was homogeneously covered by MWCNTs maintained a unique tubular structure. MWCNT-coated PLLA showed remarkable higher wettability than uncoated PLLA. Human osteosarcoma cell line (Saos2) adhered well on the CNT-coated PLLA whereas there are few cells attached on the uncoated PLLA at 2h after seeding. The number of the cells on uncoated PLLA was still smaller than on the MWCNT-coated PLLA at 1 and 3 days. Moreover, The DNA content in the cells attached to the MWCNT-coated PLLA was significantly higher than that on the uncoated PLLA (p0.1). Therefore MWCNT-coating on PLLA improved the surface wettability and initial cell attachment at early stage.