Induction of classical activation of macrophage in vitro by water soluble chitin

• Published in: Applied surface science 2012-12, Vol.262, p.134-139
• Main Authors: Jeon, Dong-Won, Ahn, Woong Shick, You, Su Jung, Chae, Gue Tae, Shim, Young Bock, Chun, Heung Jae
• Format: Article
• Language: English
• Subjects: Classical activation; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Macrophage; Physics; RAW 264.7; Water soluble chitin
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2012.03.078

► Water soluble chitin (WSC) was prepared by re-acetylation of chitosan and was treated with the murine RAW 264.7 macrophage cell lines (ATCC TIB-71). ► WSC induced classical activation in the RAW 264.7 cells, accompanied by the induction of associated genes. ► The association of histone H2A gene perturbation was found. The purpose of this study is to understand the effect of chitin on macrophage mediated immunity, which is a significant factor to wound healing and tissue regeneration. In this work, water soluble chitin (WSC) was prepared by re-acetylation of chitosan and was treated with the murine RAW 264.7 macrophage cell lines (ATCC TIB-71). WSC induced classical activation in the RAW 264.7 cells, accompanied by the induction of associated genes. The results suggest that WSC is one of the functional chitin molecules that are responsible for the immune response, especially present in macrophage classical activation.