Study of the green synthesis of silver nanoparticles using a natural extract of dark or white Salvia hispanica L. seeds and their antibacterial application

• Published in: Applied surface science 2019-09, Vol.489, p.952-961
• Main Authors: Hernández-Morales, Liliana, Espinoza-Gómez, Heriberto, Flores-López, Lucía Z., Sotelo-Barrera, Erika Lis, Núñez-Rivera, Alfredo, Cadena-Nava, Rubén Darío, Alonso-Núñez, Gabriel, Espinoza, Karla Alejandra
• Format: Article
• Language: English
• Subjects: Dark chia; Green synthesis; Salvia hispanica natural extract; Silver nanoparticles; White chia
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2019.06.031

In this research work, we report for the first time the green synthesis of silver nanoparticles (AgNPs), using two varieties of a natural extract of dark (D) or white (Wh) Salvia hispanica L. seeds, commonly named chia (Ch), as a reducing-stabilizing agent. Similarly, it was carried out a study on the effect of the variation of different physicochemical parameters, like aging time and storage temperature of the natural extract of dark Ch seeds, in the morphology, size and polydispersity of the prepared AgNPs. We found that the ideal conditions to do the green synthesis of AgNPs, are to use the Ch extract one day after it have been prepared and stored at 5 °C (1D C). The AgNPs were characterized by UV–Vis, FT-IR, FE-SEM-EDS and TEM. The TEM images of the synthesized AgNPs showed spherical morphology with an average nanoparticle size of 7 nm, at optimum conditions (AgNPs/D Ch (1D C)). Additionally, we studied the antimicrobial activity of the green synthesized AgNPs with both types of Ch seeds, against bacteria gram-positive and gram-negative, using as the bacterial model E. coli and S. aureus, respectively. We determined that the synthesized AgNPs/D Ch have more antimicrobial activity against both bacteria, in comparation with AgNPs/W Ch (ZOI against E. coli, 18.5 mm; S. aureus, 14.9 mm). [Display omitted] •We report a green chemistry method for silver nanoparticles (Ag-NPs) synthesis with Salvia hispanica seed aqueous extract.•We study the effect of the type of Salvia hispanica seed on the silver nanoparticles (Ag-NPs) synthesis.•The silver nanoparticles (Ag-NPs) characterization were done by Uv-vis, SEM-EDS, and TEM.•The nanoparticles obtained are spherical in shape and between 1 and 27 nm in size.