Laser-Mediated antibacterial effects of Few- and Multi-Layer Ti3C2Tx MXenes

• Published in: Applied surface science 2021-11, Vol.567, p.150795, Article 150795
• Main Authors: Rosenkranz, Andreas, Perini, Giordano, Aguilar-Hurtado, Jose Y., Zambrano, Dario F., Wang, Bo, Niccolini, Benedetta, Henriques, Patricia C., Rosa, Enrico, De Maio, Flavio, Delogu, Giovanni, De Spirito, Marco, Palmieri, Valentina, Papi, Massimiliano
• Format: Article
• Language: English
• Subjects: Antibacterial; MXenes; Nanomaterials; Near infra-red; Photothermal therapy
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2021.150795

[Display omitted] •Laser-mediated antibacterial effects studied for few-(FX) and multi-layer (MX) MXene.•FX and MX are biocompatible on eukaryotic cells below 100 µg/mL.•Bacteria cells are reversibly trapped in FX and MX agglomerations in solution.•Both MXenes (100 µg/mL) kill E. coli and S. aureus with 100% efficacy under irradiation.•Efficient NIR antibacterial treatment obtained with 5.7 Wcm−2 (MX) and 3 Wcm−2 (FX) Ti3C2TX nano-sheets (MXenes) with excellent light-conversion capacity have gained importance in treating infectious diseases due to their limited bacterial resistance. In this study, we exploit this property to design photothermal antibacterial therapy using few- (FX) and multi-layer (MX) Ti3C2Tx nano-sheets. We demonstrate that FX have a higher cytocompatibility and conversion of light to heat, but MX show a better efficacy in inhibiting growth of S. aureus and E. coli due to MXenes’ reversible bacteria trapping. For MX (25 µg/mL), □37% of E. coli and □23% of S. aureus cells survived, while the effect was less pronounced for FX with □72% of E. coli and □46% of S. aureus viable cells after treatment. After using 100 µg/mL of MX, □11% of E. coli and □4% of S. aureus survived, while FX had only a mild effect on both species. The NIR laser treatment increased the efficacy of both materials: 100 µg/mL of MX combined with 5 min laser treatment at 5.7 W cm − 2 completely killed both species. For FX, the treatment with 3 W cm − 2 and the highest concentration (100 µg/mL) induced an effect comparable to MX (87% on E. coli, 95% on S.aureus). The combined NIR-MXene treatment causes an irreversible cell death linked to the loss of cell integrity (DNA release quantification and bacteria debris observation).