Vibrio species are predominantly intracellular within cultures of Neoparamoeba perurans, causative agent of Amoebic Gill Disease (AGD)

Neoparamoeba perurans is a free-living protist that can cause Amoebic Gill Disease (AGD) in a number of teleost fish species and is responsible for substantial losses of farmed Atlantic salmon in various locations world-wide. The intimate relationship of the amoeba with bacteria can present challeng...

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Bibliographic Details
Published in:Aquaculture 2021-02, Vol.532, p.736083, Article 736083
Main Authors: MacPhail, David P.C., Koppenstein, Rhea, Maciver, Sutherland K., Paley, Richard, Longshaw, Matt, Henriquez, Fiona L.
Format: Article
Language:English
Subjects:
16S
Amoebic gill disease
Bacteria
Neoparamoeba peruran
Vibrio
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Summary:Neoparamoeba perurans is a free-living protist that can cause Amoebic Gill Disease (AGD) in a number of teleost fish species and is responsible for substantial losses of farmed Atlantic salmon in various locations world-wide. The intimate relationship of the amoeba with bacteria can present challenges for its laboratory culture and drug discovery programmes. Herein, we report our findings on the bacteria that live in close association with N. perurans. These include the presence of various marine bacteria, including those of the Pseudoalteromonas, Halomonas, Cellulophaga and Mesonia genera. However, next generation sequencing (NGS) identified a substantial proportion of sequences that matched with the Vibrio genus in filtered amoebae and not in the medium suggesting an intimate association between this genus and N. perurans. Fluorescence in-situ hybridization (FISH) revealed that Vibrio species are predominantly found within N. perurans. This information is important in the management and control of AGD as bacteria associated with N. perurans may have relevance to virulence and advancement of disease. •Neoparamoeba perurans, the causative agent of amoebic gill disease, cannot be dissociated from bacteria in in vitro culture•These bacteria are from a variety of different species that are normally associated with marine environments•Next generation sequencing reveals that a large percentage of bacteria in filtered amoeba are from the Vibrio species•Fluorescence In situ hybridisation verifies that Vibrio species is predominantly found within N. perurans
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2020.736083