Estrogenic and vitellogenic responses in genistein fed adult male Cyprinus carpio

• Published in: Aquaculture 2022-02, Vol.548, p.737559, Article 737559
• Main Authors: Nuzaiba, Pallath Muhammed, Varghese, Tincy, Gupta, Subodh, Sahu, Narottam Prasad, Srivastava, Prem Prakash
• Format: Article
• Language: English
• Subjects: Estrogen receptor; Fish vitellogenin; Gene expression; Genistein; Phytoestrogen
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2021.737559

A 60-day feeding trial was conducted to determine the impact of dietary phytoestrogen, genistein on inducing vitellogenesis in reproductively active male Cyprinus carpio. Adult male C. carpio were fed with graded levels of genistein, 0 (Control), 1 (Gen-1), 3 (Gen-3), 6 (Gen-6) and 9 (Gen-9) mg/100 g in fish feed. Serum levels of cholesterol, total testosterone and estradiol (E2) showed an increasing trend with increasing doses of genistein in the feed after the trial. Serum T3 level was reduced while the T4 level was induced with an increasing level of dietary genistein. Gene expression of brain and testicular aromatases were significantly reduced in Gen-9. The expression of estrogen receptor-α (erα) was upregulated, and that of erβ2 was downregulated with an increasing dose of genistein. Vitellogenin concentration was increased to its highest, 29.70 ± 2.63 μg/mL in Gen-6 and vtgb2 expression in the liver was increased to five-fold in Gen-6 & Gen-9 than that of control. The level of cortisol and the expression pattern of 20βHSD were reduced with an increasing dose of genistein. Hence, the study concluded that upregulation of the erα gene and high E2 concentration is responsible for genistein induced vitellogenesis in adult male C. carpio. The estrogenic responses in adult male C. carpio are accompanied by enhanced testosterone level and reduced activity of the steroidogenic enzyme, 20βHSD, indicating a possible delay in maturation. •The soy-phytoestrogen, genistein induced estradiol and vitellogenin level in male C. carpio.•There is no evidence of intra-testicular oocyte development in 1–9 mg/100 g genistein fed common carp.•Dietary genistein above 6 mg/100 g delayed sperm maturation in C. carpio.