Effect of lincomycin, an injectable lincosamide antibiotic, against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetic-pharmacodynamic profile

• Published in: Aquaculture 2022-02, Vol.548, p.737667, Article 737667
• Main Authors: Lee, Ji-Hoon, Seo, Jung Soo, Kim, Ga Won, Kwon, Mun-Gyeong, Kim, Do-Hyung, Park, Chan-Il, Kim, Keun Taek, Park, Jinbong
• Format: Article
• Language: English
• Subjects: Efficacy; Lincomycin HCl (LH); Olive flounder; Pharmacokinetic-pharmacodynamic profile; Streptococcosis
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2021.737667

Injectable antibiotics have some advantages over oral administration under certain conditions. However, they have not been adopted actively, especially in the aquaculture field. Here, we focused on the effect of intramuscular (i.m.) injection of lincomycin HCl (LH), a lincosamide antibiotic, on a streptococcal infection in olive flounders (Paralichthys olivaceus). In-vitro efficacy tests with 49 Streptococcus parauberis clinical isolates revealed inhibition of 32 isolates (65.3%) by 2 μg/mL LH, as the 50% minimum inhibitory concentration (MIC50). A single LH i.m. injection (10 mg/kg) in-vivo significantly reduced cumulative mortality rates against S. parauberis infection from 100% to 0–20.0% (P  MIC50 (time period remained above MIC50) values against S. parauberis. After intravenous injection, the volume of distribution (Vz) was high in olive flounder (2.44 L/kg). LH bioavailability was 88.99% following a single i.m. injection of 10 mg/kg. Analyses of muscle residue depletion demonstrated appropriate withdrawal times of 19.95 days (99% statistical tolerance limit) after a single 10 mg/kg i.m. injection. Overall, our results suggest that a single i.m. injection of LH at 10 mg/kg offers acceptable efficacy with reasonable withdrawal periods for streptococcal infection in olive flounder. •Lincomycin HCl (LH) at 10 mg/kg is effective against Streptococcus parauberis in olive flounder.•Clinical doses of LH will not affecting food safety standards.•Utilization of injectable single dose LH is clinically feasible in fish species.