Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet

• Published in: Aquaculture 2022-11, Vol.560, p.738485, Article 738485
• Main Authors: Milanović, Vesna, Cardinali, Federica, Aquilanti, Lucia, Maoloni, Antonietta, Garofalo, Cristiana, Zarantoniello, Matteo, Olivotto, Ike, Riolo, Paola, Ruschioni, Sara, Isidoro, Nunzio, Corsi, Lorenzo, Cardinaletti, Gloriana, Osimani, Andrea
• Format: Article
• Language: English
• Subjects: Edible insects; Erythromycin; qPCR; Risk assessment; Tetracyclines
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2022.738485

Siberian sturgeon (Acipenser baerii) represents a high-value fish farmed for obtaining high-priced food. In this study, the occurrence of antibiotic resistance (AR) genes encoding for resistance to antimicrobials administered in clinical practice, as aminoglycosides, β-lactams, macrolide-lincosamide-streptogramin B, tetracyclines, and vancomycin, were studied in the gut of sturgeons fed a diet containing 50% Hermetia illucens larvae by qPCR assays. Erm(A), erm(B), erm(C), tet(K), tet(M), tet(S), and tet(W) were detected in all samples. Feed and guts of sturgeons at beginning of rearing were negative for tet(O), vanA, vanB, mecA, and aac-aph. BlaZ was exclusively detected in control diet. The highest copy numbers were observed for tet(M), tet(K) and tet(S) in control diet. The copy numbers of most of the genes were higher in control diet than in diet containing 50% insect. Despite the differences in the gene copy numbers in control or insect-based diet, the results for AR genes contained in the guts of fish highlighted that, at 50% substitution level, no effect of the insect-based diet on the AR genes seems to occur in the experimental sturgeons at the end of rearing. •Antibiotic resistances in sturgeons fed Hermetia illucens-based feed were studied.•The detected genes were higher in control diet than in diet containing insect.•At 50% substitution no effect of the insect diet on the AR genes was seen in sturgeons.•The most detected genes were those for resistance to tetracycline and erythromycin.