Effect of ozone nanobubbles on the microbial ecology of pond water and safety for jade perch (Scortum barcoo)

• Published in: Aquaculture 2023-11, Vol.576, p.739866, Article 739866
• Main Authors: Huang, Qianjun, Ng, Pok Him, Marques, Ana Rita Pinheiro, Cheng, Tzu Hsuan, Man, Ka Yan, Lim, Kwok Zu, MacKinnon, Brett, Huang, Liqing, Zhang, Ju, Jahangiri, Ladan, Furtado, William, Hasib, F.M. Yasir, Zhong, Liang, Kam, Ho Yat, Lam, Chun Ting, Liu, Hong, Yang, Ying, Cai, Wenlong, Brettell, Dominic, St-Hilaire, Sophie
• Format: Article
• Language: English
• Subjects: Bacterial species; Jade perch; Metagenomics; Nanobubbles; Ozone; Pond microbial community
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2023.739866

The microbial composition and diversity in aquaculture pond ecosystems are closely related to animal productivity and disease outbreaks. Interventions that alter the bacteria found in pond water can therefore affect the productivity of these systems. Ozone nanobubbles have recently been shown to reduce pathogens, improve dissolved oxygen, and influence fish innate immunity. However, little is known about the effect of nanobubble treatment on the microbial community of aquaculture ponds. This study investigated the impact of ozone macrobubbles (O3MB) and nanobubbles (O3NB) on the microbial ecology of pond water and fish health. We successfully eliminated between 90.9 and 99.4% of the heterotrophic bacteria and 90.1 to 95.2% of the bacterial DNA in our small pond water ecosystems after treatment with 0.15 mg/L ozone. According to the shotgun metagenomic sequencing, ozone macro- and nanobbuble treatments reduced the relative abundance of all bacteria in our water sample, including the dominant bacterial species, as well as Cyanobacteria. The top ten bacterial species in the community changed and were more evenly distributed within the water sample. The bacterial richness of the ozone-treated water samples declined slightly, but over 6000 species were still identified 24 h after the treatment. We also observed a rebound in the bacterial community 24 h after the ozone treatments. The advantage of the nanobubble delivery of ozone over macrobubble delivery of this gas was that the former took significantly less time to deliver the desired quantity of gas while it also greatly increased the dissolved oxygen in the water. Further, we assessed the impact of ozone nanobubbles on jade perch, and no effects were found on the fish at an exposure dose of 0.15 mg/L. This study provides preliminary information on potential applications of nanobubble technology for “resetting” microbial communities, which may be useful during disease outbreaks. •A low dose ozone treatment eliminated up to 99.4% of the heterotrophic bacteria and 95.2% of the bacterial DNA in pond water.•Ozone treatment at 0.15mg/L was safe for Jade perch.•The bacterial community rebounded with an even distribution and a shift in the top 10 species 24 h after treatment.•Nanobubbles greatly increased the oxygen in the water and were more efficient than air stones for delivering ozone.