Distribution and environmental preferences of Saprolegnia and Leptolegnia (Oomycota) species in fish hatcheries in Hungary

• Published in: Aquaculture 2025-02, Vol.596, p.741737, Article 741737
• Main Authors: Eszterbauer, Edit, Erdei, Noémi, Hardy, Tímea, Kovács, Antónia, Verebélyi, Viktória, Hoitsy, György, Katics, Máté, Bernáth, Gergely, Lang, Zsolt, Kaján, Győző L.
• Format: Article
• Language: English
• Subjects: aquaculture; Cyprinidae; fish; Freshwater aquaculture; geographical distribution; host preferences; Hungary; ITS–rDNA phylogeny; Leptolegnia; nucleotide sequences; Oomycete; phylogeny; Salmonidae; Saprolegnia; Saprolegniosis; sequence analysis; species; taxonomic revisions; temperature; Water mold
• ISSN: 0044-8486
• DOI: 10.1016/j.aquaculture.2024.741737

We studied for the first time the abundance and species distribution of water molds (Oomycota, Saprolegniales) in fish hatcheries in Hungary, with a focus on Saprolegnia and Leptolegnia spp. A total of 182 water mold isolates were obtained from different developmental stages of fish (eggs, fry, and adults) and water samples collected during the spawning season of cyprinids and salmonids. Of the ten water mold species identified based on their ITS–rDNA sequences, S. parasitica, S. ferax, and S. australis were the most abundant. Statistical analyses of species proportions in relation to the sample source and host preference revealed that S. parasitica was significantly more abundant in the host than in water samples, whereas the opposite trend was observed for S. ferax and S. australis. In addition, our results confirmed previous findings that S. australis and S. ferax prefer warm water (+21–24 °C) and that S. parasitica has a wide temperature tolerance (+3–24 °C). The DNA sequence identity analysis showed that the species demarcation criterion for Saprolegnia spp. was approximately 98 % identity, based on ITS–rDNA sequences. Furthermore, our phylogenetic analyses suggest that taxonomic revision of Leptolegnia spp. is necessary. •First study on the species distribution of water molds on fish hatcheries in Hungary.•Differences in the abundance of water mold species in environmental factors.•Saprolegnia species demarcation criterion is 98 % identity based on ITS–rDNA.•Taxonomic revision of Leptolegnia spp. is required.