Acute toxic effects of zinc oxide nanoparticles on Hydra magnipapillata

• Published in: Aquatic toxicology 2018-12, Vol.205, p.130-139
• Main Authors: Yamindago, Ade, Lee, Nayun, Woo, Seonock, Choi, Hyosun, Mun, Ji Young, Jang, Seok-Won, Yang, Sung Ik, Anton-Erxleben, Friederike, Bosch, Thomas C.G., Yum, Seungshic
• Format: Article
• Language: English
• Subjects: Animals; DNA - drug effects; DNA repair; Endocytosis; Hydra - drug effects; Lysosome; Metal Nanoparticles - toxicity; Nanotoxicity; Signal Transduction - drug effects; Stress response; Water Pollutants, Chemical - toxicity; Zinc Oxide - toxicity
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2018.10.008

•Toxicity of the Zinc oxide nanoparticles (ZnO NPs) is influenced by the size of the particles and the period of exposure.•ZnO NPs induce changes in the morphology and abnormal regeneration in H. magnipapillata.•ZnO NPs penetrated and accumulated in the cells of H. magnipapillata.•ZnO NPs induce cytotoxic and genotoxic responses. Zinc oxide nanoparticles (ZnO NPs) are increasingly used in various products as coating and additive materials for household goods, personal-care products, and drug delivery systems. Because of their broad applications, the potential risks to nontarget organisms associated with their input into aquatic environments have generated much concern. We investigated the acute toxicity, morphological responses, and potential impact on physiology and metabolism in polyps exposed to spherical ZnO NPs of either 20 nm (ZnO NP20) or 100 nm (ZnO NP100). The median lethal concentrations (LC50) of ZnO NP20 were 55.3, 8.7, and 7.0 μg/mL after exposure for 48, 72, and 96 h, respectively; and those of ZnO NP100 were 262.0, 14.9, and 9.9 μg/mL, respectively. The morphological responses of the hydra polyps to a range of ZnO NP concentrations suggest that ZnO NPs may negatively affect neurotransmission in Hydra. ZnO NPs may also induce abnormal regeneration in the polyps by affecting the expression of several genes related to the Wnt signaling pathway. The presence of ZnO NP20 in the hydra tissue was confirmed with electron microscopy. A Gene Ontology analysis of the genes differentially expressed in hydra polyps after exposure to ZnO NP20 for 12 or 24 h revealed changes in various processes, including cellular and metabolic process, stress response, developmental process, and signaling. A KEGG pathway analysis of hydra polyps after exposure of ZnO NP20 or ZnO NP100 for 12 or 24 h demonstrated various changes, including in the DNA replication and repair, endocytosis, lysosomes, Wnt signaling, and natural killer-cell-mediated cytotoxicity pathways, suggesting the mechanisms that maintain cellular homeostasis in response to ZnO NPs. Progesterone-mediated oocyte maturation was also affected by the ZnO NPs nanoparticles, suggesting that they are potential endocrine disruptors. This study should increase our concern regarding the dispersal of ZnO NPs in aquatic environments.