μEvaluation of microcystin-LR absorption using an in vivo intestine model and its effect on zebrafish intestine

• Published in: Aquatic toxicology 2019-01, Vol.206, p.186-194
• Main Authors: Li, Jian, Chen, Chuanyue, Zhang, Tongzhou, Liu, Wanjing, Wang, Li, Chen, Yuanyuan, Wu, Lei, Hegazy, Abeer M., El-Sayed, A.F., Zhang, Xuezhen
• Format: Article
• Language: English
• Subjects: Gut microbiota; Intestine model; Microcystin-LR; Stress responses; Zebrafish
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2018.11.014

•An ex vivo everted gut sac model was applied in 6 fish to evaluate the absorption of MC-LR.•The absorption and accumulation of MC-LR were dose-dependent and positively correlated with Oatp2b1.•Zebrafish possess a core microbiota that was resistant of MC-LR.•High dose of MC-LR altered the characteristics of high abundances gut microbiota.•MC-LR increased the risks of pathogen invasion but probiotics in zebrafish intestine exhibitresistance to this trend. Microcystin-LR (MC-LR) is regarded as one of the most toxic microcystins (MCs) isoforms. Microcystins could cause multiple organs dysfunction, and more attention has been drawn to the toxic effects on the gastrointestinal disorder. By using ex vivo everted gut sac model in 6 fish (Carassius auratus, Megalobrama amblycephala, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus and Cyprinus carpio) and determining the accumulation of MC-LR in zebrafish intestine, we found a dose-dependent manner in the absorption and accumulation of MC-LR. Until now, little studies have been reported concerning the gut microbiota composition caused by different MC-LR exposure. The present study is the first time characterized the phylogenetic composition and taxonomic of the bacterial communities growth in the intestines of zebrafish treated with MC-LR using 16S rRNA pyrosequencing. After 30 days of treatment with 0, 1, 5 or 20 μg/L MC-LR, the alpha and beta diversity did not generate significant differences, indicating the existence of a core microbiota. However, db-RDA analysis showed that treatment with 20 μg/L MC-LR changed the characteristics of high abundances microbiota. The expression of Oatp2b1, stress related enzyme activities in gut and their associations with gut microbiota were also determined. The identified phylotypes including Actinobacteria, Lactobacillus and some opportunistic pathogens highlight the increasing risks of pathogen invasion and recovery tendency via potential probiotics resistance in zebrafish exposed to MC-LR.