A Study of the Effect of Proinflammatory Cytokines on the Epithelial Cells of Smokers, With or Without COPD

Abstract Introduction Cigarette smoke is the main cause of inflammation in COPD. The mechanisms that differentiate smokers who develop COPD are diverse. In this study, we analyzed the presence of cytokines in the respiratory secretions of smokers with or without COPD and the secretory properties of...

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Bibliographic Details
Published in:Archivos de bronconeumología (English ed.) 2011, Vol.47 (9), p.447-453
Main Authors: De Diego Damiá, Alfredo, Cortijo Gimeno, Julio, Selma Ferrer, M. José, León Fabregas, Montserral, Almudever Folch, Patricia, Milara Paya, Javier
Format: Article
Language:English
Subjects:
Citocinas
COPD
Cytokines
EPOC
Humo de tabaco
Inflamación
Inflammation
Internal Medicine
Pulmonary/Respiratory
Tobacco smoke
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Summary:Abstract Introduction Cigarette smoke is the main cause of inflammation in COPD. The mechanisms that differentiate smokers who develop COPD are diverse. In this study, we analyzed the presence of cytokines in the respiratory secretions of smokers with or without COPD and the secretory properties of the differentiated bronchial epithelium obtained from the individuals themselves after exposure to tobacco smoke. Material and methods Twenty-seven smokers were studied, 12 of whom had COPD that had not been previously treated with steroids. In 11, samples were obtained by means of induced sputum, and the remaining samples were collected from bronchial aspiration after bronchoscopy. Concentrations of IL8, IL13, and TNFα in the supernatant were determined. The results obtained were compared between individuals with and without COPD, and we studied their relationship with the severity of COPD as expressed by the degree of obstruction, dyspnea, presence of hypersecretion and intensity of smoking. Bronchial epithelial cell cultures were obtained by air–liquid interface in 4 smokers. The samples were exposed to increasing concentrations of cigarette smoke (5%–20%) and the epithelial mRNA expressions of Muc5AC, IL8, and TNFα were determined. Results COPD patients had significantly higher values of IL8 than healthy smokers (41 [22] pM vs 21 [12] pM). The values of IL8 correlated significantly with the severity of the obstruction ( r =0.6; P
ISSN:1579-2129
1579-2129
DOI:10.1016/j.arbr.2011.04.007