Bombyx mori cathepsin D expression is induced by high temperature and H₂O₂ exposure

Cathepsin D is involved in the metamorphosis of the silkworm, Bombyx mori. Here, we show the expression profile of B. mori cathepsin D (BmCatD) in the fat body during exposure to stressors, such as high temperature and H₂O₂. Exposure of larvae in the fifth instar stage to high temperature (28℃) led...

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Bibliographic Details
Published in:Journal of Asia-Pacific entomology 2011-09, Vol.14 (3), p.285-288
Main Authors: Kim, B.Y., Dong-A University, Busan, Republic of Korea, Lee, K.S., Dong-A University, Busan, Republic of Korea, Sohn, M.R., Dong-A University, Busan, Republic of Korea, Kim, K.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Choi, K.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kang, P.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Jin, B.R., Dong-A University, Busan, Republic of Korea
Format: Article
Language:English
Subjects:
BOMBYX MORI
Cathepsin D
ESTRES OXIDATIVO
GUSANO DE SEDA
METAMORFOSIS
METAMORPHOSE
METAMORPHOSIS
OXIDATIVE STRESS
SILKWORMS
STRESS OXYDATIF
TEMPERATURA
TEMPERATURE
VER A SOIE
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Summary:Cathepsin D is involved in the metamorphosis of the silkworm, Bombyx mori. Here, we show the expression profile of B. mori cathepsin D (BmCatD) in the fat body during exposure to stressors, such as high temperature and H₂O₂. Exposure of larvae in the fifth instar stage to high temperature (28℃) led to accelerated metamorphosis and shortened larval stage compared to control larvae grown at 23℃. Concomitantly, the expression level of BmCatD mRNA was greatly increased during exposure to high temperature. We also detected significantly elevated H₂O₂ levels in the hemolymph of larvae treated with high temperature. To confirm that oxidative stress induces BmCatD expression, B. mori larvae were injected with H₂O₂. As predicted, we observed increased expression of BmCatD following H₂O₂ exposure. Based on these results, we conclude that BmCatD expression is induced by high temperature and H₂O₂ exposure and that this stress-induced BmCatD expression leads to early metamorphosis.
ISSN:1226-8615
1876-7990
DOI:10.1016/j.aspen.2011.04.002