Aberrant antibody responses to oxidized LDL and increased intimal thickening in apoE−/− mice exposed to cigarette smoke

• Published in: Atherosclerosis 2004-07, Vol.175 (1), p.7-14
• Main Authors: Tani, Shigemasa, Dimayuga, Paul C, Anazawa, Takeo, Chyu, Kuang-Yuh, Li, Hongyan, Shah, Prediman K, Cercek, Bojan
• Format: Article
• Language: English
• Subjects: Animals; Antibodies - blood; Antioxidants - analysis; Apolipoproteins E - deficiency; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Blood coagulation. Blood cells; Carboxyhemoglobin - analysis; Cardiology. Vascular system; Carotid Arteries - pathology; CD4-CD8 Ratio; Cholesterol - blood; Cytokines - metabolism; Fundamental and applied biological sciences. Psychology; General and cellular metabolism. Vitamins; Immune response; Immunoglobulin G - blood; Immunoglobulin M - blood; Intimal thickening; Lipoproteins, LDL - immunology; Male; Medical sciences; Mice; Mice, Knockout; Molecular and cellular biology; Nitric Oxide - blood; Oxidized LDL; Pharmacology. Drug treatments; Phosphorylcholine - immunology; Platelet; Signal Transduction; Smoking; Spleen - immunology; Spleen - metabolism; Tobacco Smoke Pollution; Tunica Intima - pathology
• ISSN: 0021-9150; 1879-1484
• DOI: 10.1016/j.atherosclerosis.2004.01.035

Cigarette smoking is associated with increased atherosclerosis and intimal thickening, and has immune-suppressive effects. The immune system modulates atherosclerosis and intimal thickening. We hypothesized that detrimental effects of cigarette smoke (CS) involves modulation of the immune response to oxidized low-density lipoprotein (oxLDL). ApoE−/− mice fed Western diet were exposed to CS starting at 20 weeks of age. Control mice were exposed to air. After 5 weeks of CS, mice were subjected to carotid arterial cuffing for 21 days. Intimal thickening was significantly increased in CS mice compared to control (0.050±0.034 mm 2 versus 0.023±0.021 mm 2; P