Accumulation of overoxidized Peroxiredoxin III in aged rat liver mitochondria

Overoxidation and subsequent inactivation of Peroxiredoxin III (PrxIII), a mitochondrial H2O2 scavenging enzyme, have been reported in oxidative stress conditions. No data are available in the literature about the presence of overoxidized forms of PrxIII in aged tissues. Liver mitochondria from 12-m...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2009-07, Vol.1787 (7), p.890-896
Main Authors: Musicco, Clara, Capelli, Valentina, Pesce, Vito, Timperio, Anna Maria, Calvani, Menotti, Mosconi, Luigi, Zolla, Lello, Cantatore, Palmiro, Gadaleta, Maria Nicola
Format: Article
Language:English
Subjects:
Acetylcarnitine - pharmacology
Aging
Animals
Cysteine - chemistry
Cysteine - metabolism
Electrophoresis, Gel, Two-Dimensional
Male
Mass Spectrometry
Mitochondria, Liver - metabolism
Molecular Weight
Nootropic Agents - pharmacology
Oxidation-Reduction
Peroxiredoxin III
Peroxiredoxins - chemistry
Peroxiredoxins - genetics
Peroxiredoxins - metabolism
Protein oxidation
Rat liver mitochondria
Rats
Rats, Inbred F344
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Two-dimensional electrophoresis
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Summary:Overoxidation and subsequent inactivation of Peroxiredoxin III (PrxIII), a mitochondrial H2O2 scavenging enzyme, have been reported in oxidative stress conditions. No data are available in the literature about the presence of overoxidized forms of PrxIII in aged tissues. Liver mitochondria from 12-month-old rats and 28-month-old rats were here analyzed by two-dimensional gel electrophoresis. A spot corresponding to the native form of PrxIII was present in adult and old rats with the same volume, whereas an additional, more acidic spot, of the same molecular weight of the native form, accumulated only in old rats. The acidic spot was identified, by MALDI-MS analysis, as a form of PrxIII bearing the cysteine of the catalytic site overoxidized to sulphonic acid. This modified PrxIII form corresponds to the irreversibly inactivated enzyme, here reported, for the first time, in aging. Three groups of 28-month-old rats treated with acetyl-l-carnitine were also examined. Reduced accumulation of the overoxidized PrxIII form was found in all ALCAR-treated groups.
ISSN:0005-2728
0006-3002
1879-2650
DOI:10.1016/j.bbabio.2009.03.002