Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function

• Published in: Biochimica et biophysica acta 2016-01, Vol.1857 (1), p.34-45
• Main Authors: Pokorná, Lucia, Čermáková, Petra, Horváth, Anton, Baile, Matthew G., Claypool, Steven M., Griač, Peter, Malínský, Jan, Balážová, Mária
• Format: Article
• Language: English
• Subjects: Cardiolipins - biosynthesis; Electron Transport Complex IV - metabolism; Mitochondria; Mitochondria - metabolism; Mitochondria - ultrastructure; Morphology; Phosphatidylglycerol; Phosphatidylglycerols - metabolism; Phospholipases - physiology; Respiration; Saccharomyces cerevisiae - metabolism; Yeast
• ISSN: 0005-2728; 0006-3002; 1879-2650
• DOI: 10.1016/j.bbabio.2015.10.004

In yeast, phosphatidylglycerol (PG) is a minor phospholipid under standard conditions; it can be utilized for cardiolipin (CL) biosynthesis by CL synthase, Crd1p, or alternatively degraded by the phospholipase Pgc1p. The Saccharomyces cerevisiae deletion mutants crd1Δ and pgc1Δ both accumulate PG. Based on analyses of the phospholipid content of pgc1Δ and crd1Δ yeast, we revealed that in yeast mitochondria, two separate pools of PG are present, which differ in their fatty acid composition and accessibility for Pgc1p-catalyzed degradation. In contrast to CL-deficient crd1Δ yeast, the pgc1Δ mutant contains normal levels of CL. This makes the pgc1Δ strain a suitable model to study the effect of accumulation of PG per se. Using fluorescence microscopy, we show that accumulation of PG with normal levels of CL resulted in increased fragmentation of mitochondria, while in the absence of CL, accumulation of PG led to the formation of large mitochondrial sheets. We also show that pgc1Δ mitochondria exhibited increased respiration rates due to increased activity of cytochrome c oxidase. Taken together, our results indicate that not only a lack of anionic phospholipids, but also excess PG, or unbalanced ratios of anionic phospholipids in mitochondrial membranes, have harmful consequences on mitochondrial morphology and function. •Phosphatidylglycerol utilized by Crd1p is protected from specific degradation.•Mitochondrial morphology is abnormal in yeast with high phosphatidylglycerol.•High phosphatidylglycerol content correlates with increased respiration.•Activity of cytochrome c oxidase is increased upon deletion of the PGC1 gene.