H3 mRNA level as a new proliferative marker in astrocytomas

Replication-dependent H3.1 and H3.2 histones are encoded by 11 genes. The H3 mRNA levels in brain astrocytomas using real-time RT-PCR assay was examined. The sequence of primers and probe used in amplification was designed basing on the reference sequence GenBank accession no. Z83737 . The H3 mRNA l...

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Published in:Biochimica et biophysica acta 2004-05, Vol.1689 (1), p.42-46
Main Authors: Orchel, Joanna, Slowinski, Jerzy, Mazurek, Urszula, Wilczok, Tadeusz
Format: Article
Language:English
Subjects:
Adult
Aged
Alternative Splicing
Astrocytoma
Astrocytoma - genetics
Astrocytoma - pathology
Base Sequence
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Cell Division
Histone gene
Histones - genetics
Humans
Ki-67 Antigen - analysis
Ki-67 Antigen - metabolism
Middle Aged
Molecular Sequence Data
Neoplasm Staging
Prognosis
Proliferation
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
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Summary:Replication-dependent H3.1 and H3.2 histones are encoded by 11 genes. The H3 mRNA levels in brain astrocytomas using real-time RT-PCR assay was examined. The sequence of primers and probe used in amplification was designed basing on the reference sequence GenBank accession no. Z83737 . The H3 mRNA levels correlated with tumor grade ( R=0.56, P=0.0012), Ki-67 proliferative antigen labeling index ( R=0.58, P=0.0008) and patient survival time ( R=−0.50, P=0.005), discriminating low-grade and high-grade tumors. Quantification of H3 mRNA with real-time RT-PCR using the proposed pair of primers may supplement classic proliferative tests and predictive factors in brain astrocytomas.
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/j.bbadis.2004.01.006