Complete RNAi rescue of neuronal degeneration in a constitutively active Drosophila TRP channel mutant

RNA interference has been widely used to reduce the quantity of the proteins encoded by the targeted genes. A constitutively active, dominant allele of trp, Trp P365 , causes massive degeneration of photoreceptors through a persistent and excessive Ca 2+ influx. Here we show that a substantial reduc...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta 2004-09, Vol.1674 (1), p.91-97
Main Authors: Geng, Chaoxian, Pellegrino, Ann, Bowman, John, Zhu, Liqin, Pak, William L.
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Calcium Channels - genetics
Calcium Channels - metabolism
Drosophila
Drosophila melanogaster - cytology
Drosophila melanogaster - physiology
Electroretinography
Humans
Ion channel hypothesis
Mutation
Neuronal degeneration
Neurons - metabolism
Neurons - pathology
Photoreceptor Cells, Invertebrate - cytology
Photoreceptor Cells, Invertebrate - metabolism
RNA Interference
RNAi rescue
Transgenes
TRP channel
TRPC Cation Channels
Unregulated Ca 2+ influx
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RNA interference has been widely used to reduce the quantity of the proteins encoded by the targeted genes. A constitutively active, dominant allele of trp, Trp P365 , causes massive degeneration of photoreceptors through a persistent and excessive Ca 2+ influx. Here we show that a substantial reduction of the TRP channel protein by RNAi in Trp P365 heterozygotes completely rescues the neuronal degeneration and significantly improves the light-elicited responses of the eye. The reduction need not be complete, suggesting that rescue of degeneration may be possible with minimal side effects arising from overdepletion of the target protein.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2004.06.002